Summary

Despite numerous measures taken at national and international levels, the status of Danube sturgeon populations has worsened dramatically, urging their protection at basin level to prevent extinction. In this context, the identification of functional population segments (management entities) is considered of highest priority for sustainable fisheries and conservation management. Thus, the present project aims to develop and apply new genetic markers (microsatellite loci and mitochondrial DNA) to determine the stock structure and to verify and distinguish between long and medium distance migrants of the spring and fall races of the four sturgeon species Acipenser gueldenstaedtii,  A. ruthenusA. stellatus and Huso huso in the Romanian part of the Danube. The same methods will be used in Turkey to discriminate the fish that are endemic in rivers Yesilırmak, Kizilırmak, Çoruh and Sakarya from fish that are utilizing Turkish coastal waters as foraging grounds solely but originate from Western and Northern Black Sea tributaries including the Danube River. Data on the stock structure and genetic differentiation between them will finally be used to define management entities and to provide recommendations for fisheries as well as conservation management including captive breeding schemes that maximize genetic variability of stocks while maintaining their genetic integrity.

Short Profile

Duration

  • 01.04.2015 – 31.10.2018

Partners

Team

About

Work packages (WP)

  • WP1: Sample collection in Romania and Turkey
    Partner: DDNI and CFRI, IUFF
  • WP2: Isolation of species-specific microsatellite loci
    Partner: IGB (subcontracting a commercial service: GenoScreen, France, www.genoscreen.com )
  • WP3: Developing panels of 12-15 microsatellite loci per species for routine genotyping
    Partner: IGB
  • WP4: Microsatellite genotyping of Romanian sturgeon samples
    Partner: IGB and DDNI
  • WP5: Microsatellite genotyping of Turkish sturgeon samples
    Partner: CFRI and IGB
  • WP6: Developing species-specific primers for sequencing the target mtDNA regions
    Partner: DDNI
  • WP7: Mitochondrial DNA sequencing of Romanian and Turkish sturgeon samples
    Partner: DDNI and CFRI
  • WP8: Population genetic analyses of data; identification of management units
    All partners

Short Profile

Duration

  • 01.04.2015 – 31.10.2018

Department

  • Laboratories and Research Stations
  • Genetics and Molecular Ecology Laboratory

Team

Partners

Funded by

This project is conducted within the ERA-Net COFASP (Cooperation in Fisheries, Aquaculture and Seafood Processing). The German partner (IGB) is financially supported by the German Federal Ministry of Food and Agriculture (BMEL) through the Federal Office for Agriculture and Food (BLE), grant number 2814ERA01G. The Romanian partner (DDNI) is financially supported by the Executive Agency for Higher Education, Research, Development and Innovation Funding (UEFISCDI grant number 26 / 2015). The Turkish partners (CFRI und IUFF) are financially supported by the Republic of Turkey, Ministry of Food, Agriculture and Livestock.

Publication

Papers

Kohlmann, K., Kersten, P., Geßner, J., Onără, D., Taflan, E. and Suciu, R., 2017. Isolation and characterization of 18 polymorphic microsatellite markers for the critically endangered stellate sturgeon, Acipenser stellatus. European Journal of Wildlife Research, 63(5), p.75.   Kohlmann, K., Kersten, P., Geßner, J., Onără, D., Taflan, E. and Suciu, R., 2017. New microsatellite multiplex PCR sets for …

Contact

Institution

“DANUBE DELTA” NATIONAL INSTITUTE FOR RESEARCH AND DEVELOPMENT

Headquarter: 165 Babadag street, Tulcea 820112, Romania

 

  • Working Hours: Monday – Thursday: 8.00 – 16.30 / Friday – 8.00 – 14.00
  • Phone (Secretary Office): (+4) 0240 – 531 520
  • Alternative phone numbers: (+4) 0240 – 524 546 / 524 548 / 524 550
  • Fax: (+4) 0240 – 533 547
  • e-mail: office@ddni.ro
  • http://www.ddni.ro

Contact person

Radu Suciu

Project Coordinator

Department Laboratories and Research Stations

  • Email: radu.suciu@ddni.ro
  • Phone: +40 (0)751 179 168
  • Fax: +40 (0)240 533 547
  • Address: Babadag Str. 165, 820 112 Tulcea, RO

Working group

  • Sturgeon Research Group
  • Genetics and Molecular Ecology